How Membrane Protein Sizing Is Used In Research

Researchers that study the characteristics of membranes need to thoroughly study the molecular structure of the membrane before they conduct experiments or studies on it. Cellular membranes are made up of a bilayer that contains mostly phospholipids. Proteins too form an integral part of membranes, and researchers can gather a lot of important data by conducting experiments onmembrane protein sizing if they have appropriate equipment at hand.

There are 3 different types of proteins in membranes. One major type of protein is Integral Membrane Protein or IMP that covers the entire bilayer and usually helps out in transport and attachment activities. These proteins could also cover the membrane more than once. The other types of proteins are Peripheral Membrane Proteins or PMP that are normally attached to the IMP proteins by forming hydrogen bonds on either ends of their surface. PMP proteins too usually help in attachment activities. The last types of proteins are known as Lipid Anchored Proteins or LAP and these are anchored to the membrane lipids, again on either faces. LAP proteins usually help in signaling and other special functions. Researchers need to bear all these features in mind when conducting research on membrane protein sizing.

The experiments and research related to proteins in membranes is especially helpful to several industries including medical, microbiology, pharmaceutical, etc. However, in order to get accurate results that could result in improved products being released in the future, it is vital that researchers have equipment that can record and store data with extreme precision. There are select manufacturers that have designed and manufactured high-precision measuring instruments for membrane protein sizing. The Paraytec ActiPix TDA 200 HT is one such model that is equipped with a precise nano-injector along with an autosampler and their futuristic ActiPix detector to collect the required data. The machine has the ability to store samples within the autosampler before injecting it into a fused capillary with the help of the integrated nano-injector that pushes 20 to 100 nL of that sample into the capillary unit.

There are dual detections done by this membrane protein sizing machine. Both the first and the second tests record the UV absorption of the sample with the unique detector of the ActiPix, known as the D 100 detector. These two tests will indicate similar peaks although the widths of those peaks will differ. The second reading will not only be wider at the peak but will also be of lower amplitude due to Taylor dispersion. These peaks are then subjected to a detailed analysis with the help of pre-programmed software that is present in the ActiPix TDA 200 system. Standard deviations are used by the analyzer to determine the hydrodynamic radius of the sample. Researchers can make their choice after studying their own needs and comparing the features offered by such manufacturers.

In order to conduct tests and experiments on membrane protein sizing with a very high level of accuracy and records statistics at a nano-level it is vital for
researchers to have the best testing equipment in their laboratory. This will enable them to understand the properties of proteins in membranes at a deeper level and come up with the desired solutions based on the data collected by such equipment.

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